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PI

研究室主宰者

研究業績

PUBLICATIONS

これまでに、細胞培養環境の時空間制御を実現するマイクロ流体技術の開発研究に従事し、iPS細胞、神経細胞、がん細胞の研究ツールとなるマイクロデバイスを開発して参りました。また、次世代の再生医療や創薬のツールとして期待されるiPS細胞由来の末梢神経束状組織の形成技術に関する研究も行っております。

査読付論文

  1. Ohka, S., Tan, S. H., Kaneda, S., Fujii, T., and Schiavo, G., “Retrograde axonal transport of poliovirus and EV71 in motor neurons” Biochem. Biophys. Res. Commun., Vol. 626, Issue 20, pp. 72-78, 2022.
  2. Mitsuzawa, S., Suzuki, N., Akiyama, T., Ishikawa, M., Sone, T., Kawada, J., Funayama, R., Shirota, M., Mitsuhashi, H., Ikeda, K., Shijo, T., Ohno, A., Nakamura, N., Ono, H., Ono, R., Osana, S., Nakagawa, T., Nishiyama, A., Izumi, R., Kaneda, S., Ikeuchi, Y., Nakayama, K., Fujii, T., Warita, H., Okano, H., and Aoki, M., “Reduced PHOX2B stability causes axonal growth impairment in motor neurons with TARDBP mutations,” Stem Cell Reports, Vol. 16, Issue 6, 1527-1541., 2021.
  3. Kaneda, S., Kurihara, K., Suzuki, K., Takanobu, H., and Ohka, S., “Microfluidic devices for the generation of centimeter-long motor nerve organoids derived from iPSCs,” Micro & Nano Letters, 15(11), pp. 746–750, 2020.
  4. Kaneda, S., Kawada, J., Shinohara, M., Kumemura, M., Ueno, R., Kawamoto, T., Suzuki, K., Kim, B. J., Ikeuchi, Y., Sakai, Y., Collard, D., Fujita, H. and Fuji, T., “Boyden chamber-based compartmentalized tumor spheroid culture system to implement localized anticancer drug treatment,” Biomicrofluidics, 13, 054111, 2019 . 掲載誌Editor’s pick articleおよび米国物理協会のShowcasing articleに選定され科学情報雑誌Scilight誌に本論文の記事が掲載
  5. Akiyama, T., Suzuki, N., Ishikawa, M., Fujimori, K., Sone, T., Kawada, J., Funayama, R., Fujishima, F., Mitsuzawa, S., Ikeda, K., Ono, H., Shijo, T., Osana, S., Nakagawa, T., Kitajima, Y., Nishiyama, A., Izumi, R., Okada, Y., Kaneda, S., Ikeuchi, Y., Mitsuhashi, H., Nakayama, K., Fujii, T., Warita, H., Okano, H., and Aoki, M., “Aberrant axon branching via Fos-B dysregulation in FUS-ALS motor neurons,” EBioMedicine, 45 362-378, 2019.
  6. Hayashi, K., Kumemura, M., Kaneda, S., Menon, V., Jalabert, L., Tachikawa, S., Tarhan, M. C., Fujii, T. Kim, B. J., and Fujita, H., “Optimization of Surface Treatment on MEMS Probes for Single-Cell Capture and Release,” Sensors and Materials, 31 9 2873-2881, 2019.
  7. Tachikawa, S., Kaneda, S., Kumemura, M., Sato, R., Tsukamoto, T., Fujii, T., Suzuki, T., and Fujita, H., “Microfluidic Device Fabricated by Three-Dimensional Lithography for Observation of Cancer Cell Invasion Process,” Electronics and Communications in Japan, 207 1 55-61, 2019.
  8. 立川 冴子, 金田 祥平, 久米村 百子, 佐藤 竜偉, 塚本 拓野, 藤井 輝夫, 鈴木 孝明, 藤田 博之, “三次元立体露光法を用いて作製したがん細胞浸潤過程観察用マイクロ流体デバイス,” 電気学会論文誌E(センサ・マイクロマシン部門誌), 138 9 435-440, 2018
  9. Kaneda, S., Kawada, J., Akutsu, H., Ichida, J., Ikeuchi, Y., and Fujii, T., “Compartmentalized embryoid body culture for induction of spatially patterned differentiation,” Biomicrofluidics, 11 041101, 2017. 掲載誌Editor’s pick articleに選定.
  10. Kawada, J., Kaneda, S., Kirihara, T., Maroof, A., Levi, T., Eggan, K., Fujii, T. and Ikeuchi, Y., “Generation of a Motor Nerve Organoid with Human Stem Cell-Derived Neurons,” Stem Cell Reports, 9 5 1441-1449, 2017.
  11. Kobayashi, M., Kim, S. H., Nakamura, H., Kaneda, S., and Fujii, T., “Cancer cell analyses at the single cell-level using electroactive microwell array device,” PLoS ONE, 10 11 e0139980, 2015.
  12. Kim, S. H., Antfolk, M., Kobayashi, M., Kaneda, S., Laurell, T., and Fujii, T., “Highly efficient single cell arraying by integrating acoustophoretic cell pre-concentration and dielectrophoretic cell trapping,” Lab on a Chip, 15 4356-4365, 2015.
  13. Kim, S. H., He, XM., Kaneda, S., Kawada, J., Fourmy, D., Noji, H., Fujii, T., Quantifying genetically inserted fluorescent protein in single iPS cells to monitor Nanog expression using electroactive microchamber arrays, Lab on a Chip, 14 4 730-736, 2014.
  14. Chowdhury, MM., Danoy, M., Rahman, F., Shinohara, M., Kaneda, S., Shiba, K., Fujita, N., Fujii, T., Sakai, Y., “Adhesion of Pancreatic Cancer Cells in a Liver-Microvasculature Mimicking Coculture Correlates with Their Propensity to Form Liver-Specific Metastasis In Vivo,” BioMed Research International, 2014 241571, 2014.
  15. Ono, K., Kaneda, S., and Fujii, T., “Single-step CE for miniaturized and easy-to-use system,” Electrophoresis, 6 6 903-910, 2013.
  16. Yamaoka, S., Ito, N., Ohka, S., Kaneda, S., Nakamura, H., Agari, T., Masatani, T., Nakagawa, K., Okada, K., Okadera, K., Mitake, H., Fujii, T., Sugiyama, M., “Involvement of the Rabies Virus Phosphoprotein Gene in Neuroinvasiveness,” Journal of Virology, 87 22 12327-12338, 2013.
  17. Nojima, T., Kaneda, S., Kimura, H., Yamamoto, T., and Fujii, T., “Application of cell-free expression of GFP for evaluation of microsystems,” Frontiers in Bioscience, 7 1931-1939, 2012.
  18. Kaneda, S., Ono K., Fukuba, T., Nojima, T., Yamamoto, T., and Fujii, T., Modification of the Glass Surface Property in PDMS-Glass Hybrid Microfluidic Devices, Analytical Sciences, 28 1 39-44, 2012.
  19. Desbois, L., Padirac, A., Kaneda, S., Genot, A. J., Rondelez, Y., Hober, D., Collard, D., and Fujii, T., “A microfluidic device for on-chip agarose microbead generation with ultralow reagent consumption,” Biomicrofluidics, 4 044101, 2012.
  20. Kaneda, S., Ono K., Fukuba, T., Nojima, T., Yamamoto, T., and Fujii, T., “A Rapid Method for Optimizing Running Temperature of Electrophoresis through Repetitive On-Chip CE Operations,” International Journal of Molecular Sciences, 12 7 4271-4281, 2011.
  21. 木村 良範, 金田 祥平, 藤井 輝夫, 村田 智, “DNAを用いたフォトニック結晶の階層的結晶成長制御,” 電気学会論文誌E(センサ・マイクロマシン部門誌), 131 8 286-291, 2011.
  22. Fukuba, T., Miyaji, A., Okamoto, T., Yamamoto, T., Kaneda, S., and Fujii, T., Integrated in situ genetic analyzer for microbiology in extreme environments, RSC Advances, 1 8 1567-1573, 2011.
  23. Ono, K., Kaneda, S., Shiraishi, T., and Fujii, T., “Optofluidic tweezer on a chip,” Biomicrofluidics, 4 4 043012, 2010.
  24. Kaneda, S., Ono, K., Fukuba, T., Nojima, T., Yamamoto, T., and Fujii, T., “Pneumatic handling of droplets on-demand on a microfluidic device for seamless processing of reaction and electrophoretic separation,” Electrophoresis, 31 22 3719-3726, 2010. 科学ニュースVerticalNewsに掲載
  25. Nojima, T., Kaneda, S., and Fujii, T., “On-chip capillary electrophoresis fractionation of DNA construct for cell-free protein expression,” Chemistry Letters, 36 11 1346-1347, 2007.
  26. Somei, K., Kaneda, S., Fujii, T., and Murata, S., “A Microfluidic Device for DNA Tile Self-Assembly,” Lecture Notes in Computer Science, 3892 325-335, 2006.
  27. Kinoshita H., Kaneda, S., Fujii T., and Oshima M., “Three-dimensional measurement and visualization of internal flow of a moving droplet using confocal micro-PIV,” Lab on a Chip, 7 3 338-346, 2005.

産業財産権

  1. 細胞培養装置 (特許第6968381号)
  2. 神経細胞を培養する装置、神経細胞を培養する方法、培養された神経細胞、軸索束内のプロテインを解析及び同定する方法並びに神経細胞の使用方法 (特許第6430680)
  3. 目的細胞の分離方法 (特許第6198186号)
  4. 生物学的対象物の分化制御方法及びその装置 (特許第5943456)
  5. 電気泳動用チップ及びこれを用いた試料の分析方法 (特許第4366523号)
  6. 分析用チップ及び分析装置並びに分析方法 (特許第4264276)
  7. マイクロチップ (特許第3977314)